Journal: The Plant Cell

Article Title: Phosphorylation of Phytochrome B Inhibits Light-Induced Signaling via Accelerated Dark Reversion in Arabidopsis ^{[W] [OA]}

doi: 10.1105/tpc.112.106898

Figure Lengend Snippet: Phenotypic Characterization of Seedlings Expressing phyBSer86Ala-YFP and phyBSer86Asp-YFP. (A) Hypocotyl growth inhibition of phyBSer86Ala-YFP seedlings exhibits extreme hypersensitivity to R light. Nontransgenic wild-type (Col-0) (closed square), and phyB-9 (cross) as well as transgenic phyB-9 seedlings expressing the phyB-GFP (closed triangle), phyBSer86Asp-YFP (open circle), and phyBSer86Ala-YFP (open triangle) fusion proteins were grown for 4 d at 22°C in darkness or at the indicated fluence rates of cR light on wet filter paper. Hypocotyl length was determined using the MetaMorph image analysis software, and the fluence rate response is shown as relative hypocotyl length to dark-grown samples (n = 50). Error bars indicate se. (B) Cotyledon expansion is impaired in transgenic seedlings expressing the phospho-mimic phyBSer86Asp-YFP grown under low-intensity R light. Nontransgenic wild-type (Col-0; 1) and phyB-9 (2) as well as transgenic phyB-9 seedlings expressing the phyB-GFP (3), phyBSer86Ala-YFP (4), and phyBSer86Asp-YFP (5) fusion proteins were grown for 4 d on Murashige and Skoog medium without sugar at 1 µmol m−2 s−1 R light. The cotyledon surface area was measured using MetaMorph image analysis software (n = 40, error bars represent se). Asterisks indicate significant difference from the wild type as determined by Student’s t test (P < 0.001). (C) Transgenic seedlings expressing the phospho-mimic phyBSer86Asp-YFP display hyposensitivity to shade. Nontransgenic wild-type (1) and phyB-9 (2) as well as transgenic P35S:PHYB-GFP (3), P35S:PHYBSer86Ala-YFP (4), and P35S:PHYBSer86Asp-YFP (5) expressing phyB-9 seedlings were grown for 7 d in high R/FR or for 3 d in high R/FR followed by 4 d in low R/FR at 20°C. The experiment was performed under constant light conditions (PAR = 130 µmol m−2 s−1). Hypocotyl lengths shown were measured using ImageJ software (n = 31 to 40). Error bars indicate se.

Article Snippet: The cotyledon surface area was measured using MetaMorph image analysis software ( n = 40, error bars represent se ).

Techniques: Expressing, Inhibition, Transgenic Assay, Software